Instructions With the new All-in-One Testing Soltion
On May 15th, 2014, the test kit started shipping with an All-in-One Testing Solution. Below are the instructions for testing nicotine strength using this new Test Solution. If you are looking for the original instructions using Bromothymol Blue and .12N Sulfuric Acid
please click here.
********** A full detailed video of this test can be found on
-eLiquid Nicotine Test Kit which contains:
(2) 3ml syringe with 0.1ml graduations.
A white 16 oz plastic cup.
All-in-One Test Solution.
Note: The calculations in the instructions only work for the All-in-One Test Solution. They will not work for .10N acid or .12N acid and Bromothymol Blue.
Rinse all supplies well with water a couple times.
Use a small accurate syringe to measure EXACTLY 1ml, 2ml or 3ml of eliquid. (detach the needle from the syringe when measuring the eliquid)
See next section for more information on which amount to use.
Put the measured amount of eliquid into your cup.
Add about 10-15ml distilled water to the cup. This amount does not need to be precise.
Swirl solution in cup to mix well.
Using a new/clean syringe, with a small gauge needle attached, measure EXACTLY 3ml of the All-in-One Test Solution. Tap out any air bubbles in the syringe for more accurate reading. (Do not use the same syringe you used to measure your eliquid)
Slowly add the test solution from the syringe to the nic solution, stopping and swirling the cup in between little adds at a time. (When you first add the test solution to the nicontine it will turn blue, but as you add more it will go from blue to green to yellow).
When you are getting to the yellowish-green color, the test end is near, so now its time to get more precise when adding the test solution via the syringe 1 very small drop at a time.
Keep repeating step 7; add then Swirl, until the solution turns all yellow.
Make note of how much test solution is left in the syringe. Subtract that number from the starting amount of test solution originally in the syringe (3ml). This is how much test solution you added. (note: if you had to 'refill' the syringe because you ran out during the test, don't forget that amount) (example: I'm using a 3.0ml syringe. I measured EXACTLY 3.0ml of test solution and used all of it in the mixture. I added EXACTLY 3.0ml more test solution to the syringe. Adding the test solution, the mixture turned yellow, and there was 1.8 ml of test solution left in the syringe. So I used a total of 4.2ml of test solution in this test)
-If you used 1ml of eliquid, Multiply this number by 17.4.
-If you used 2ml of eliquid, Multiply this number by 8.7.
-If you used 3ml of eliquid, Multiply this number by 5.8.
-Or compare that amount to this
The resulting number is the mg/ml. (example: 4.2x17.4 = 73mg/ml. ... round to the nearest whole number)
Testing 1ml of eliquid carries a +/- 3-4mg margin of error, and is recommended testing eliquid strength greater than 36mg. (uses least amount of test solution)
Testing 2ml of eliquid carries a +/- 2-3mg margin of error, and is recommended testing eliquid strength 18mg-36mg.
Testing 3ml of eliquid carries a +/- 1-2mg margin of error, and is recommended testing eliquid strength up to 18mg. (uses most amount of test solution)
When adding the test solution and mixing, the color will go from blue to bluish-green to green, to yellowish-green, to yellow with still a tint of green, and when you've reached the 'magic drop', it will turn all yellow. If you are using a clear glass, place it on a white piece of paper to see the color change easier (not needed if using a white plastic cup). Using a white plastic cup makes seeing the color change easy.
If you are testing 1ml of NIC Juice, then with about every 1 ml of test solution added, that will be around 18mg of nicotine be tested. So if you are testing eliquid that should be 50mg, then it is pointless to repeat steps 7-9, a little bit at a time in the beginning. I’ll bulk add like ml of test solution initially, then mix, then do it at 1 or 2 drops at a time after that.
If you are testing 2ml-3ml of NIC Juice, then with about every 1 ml of test solution added, that will be around 6-9mg of nicotine be tested. So if you are testing eliquid that should be 24mg, then it is pointless to repeat steps 7-9, a little bit at a time in the beginning. I’ll bulk add like 2ml of test solution initially, then mix, then do it at 1 or 2 drops at a time after that.
You can do the test a few times and average out the results if needed. As you do the test several times, you’ll get a feel for it, and become more accurate.
Before using your syringes, put some clear nail polish over the black numbers, and let dry. This will protect the markings so they don't eventually wipe off after several cleanings.
The All-in-One test solution is designed for use by average home owners, and not super dangerous. The test solution is very diluted, so it won't eat holes in things. If you get it on your skin, it might itch. If you get under your fingernails it can sting. Just wash your hands with water.
Test Solution will stain anything it gets on especially on porous counter tops/wood/etc, and clothes.
The real danger of testing is posed by the e-liquid. If you're testing high nicotine content liquid, a ml would be enough to kill children, pets, animals, even you. If you're working with very high strength, I'd caution you to use latex, rubber, or vinyl gloves and do the test in a well ventilated area. (But if you make your own juices, then you already know this)
Disposal of solution after test you should be fine pouring it in the drain, so long as you flush the drain after with some water.
Rinse your testing equipment clean with plain old tap water, shake off excess water, and let air dry. The liquid does get sticky, and gets hard to clean, if left to dry on.